起垄沟播和常规平播下滴灌棉田土壤水盐的运移

为明确滴灌条件下植棉方式对土壤水盐运移的影响，采用田间调查与室内分析相结合的方法，在滨海重度盐碱地开展了起垄沟播和常规平播植棉方式下的水盐运移试验，调查了滴灌前后两种植棉方式不同点位及不同土壤深度的土壤水分、盐分和土壤溶液电导率等指标，分析不同植棉方式土壤水分、盐分和土壤溶液电导率的时空分布特征。结果表明：滴灌条件下起垄沟播的水分入渗深度和盐分淋洗深度均明显大于常规平播植棉方式，起垄沟播植棉膜下(0~20 cm)土壤溶液电导率明显低于常规平播植棉；滴灌对两种种植方式膜外土壤水分和盐分运移未产生明显影响。起垄沟播联合滴灌技术更有利于为棉花生长的水盐环境。研究结果可为盐碱地植棉提供理论参考和实践依据。     

蔬菜中农药残留检测方法变更的验证方法探析

检测标准发生变更时,检验检测机构应做好新方法的验证工作。以水果和蔬菜中阿维菌素残留量测定标准变更为实例,详述了如何开展方法验证,以期为大家开展化学分析方法发生变更时的验证工作提供参考。     

永兴县冰糖橙果园土壤肥力特征及其综合评价

为探究永兴县冰糖橙果园土壤肥力特征及其综合性状，为果园土壤改良和合理施肥提供依据。在永兴县冰糖橙主产区采集了284处具有代表性果园表层土壤(0-40cm)，测定了土壤有机质、pH值、全氮、碱解氮、有效磷、速效钾、缓效钾等养分指标，运用模糊综合评判法，对永兴县冰糖橙果园及其北、中和南3个区域土壤肥力质量进行了综合评价，并比较3个区域的土壤肥力差异特征。结果表明：永兴县冰糖橙果园土壤肥力为中等水平，土壤为酸性，平均仅5.47；有机质含量较丰富，为较高等水平，平均为25g/kg；土壤全氮含量为较高等水平，平均为1.86g/kg，碱解氮含量为中等水平，平均109mg/kg；有效磷平均含量25.2mg/kg，为中等水平；缓效钾含量偏低，为较低等水平，平均132mg/kg；速效钾含量处于较高等水平，平均153mg/kg。果园的3个区域土壤综合肥力属于中等偏下水平，受地形地貌、成土母质、果园管理及其他因素影响，中部区果园综合肥力高于北部和南部果园。     

水稻赖氨酸含量测定方法改进及其最优动态分类研究

赖氨酸是水稻营养成分中第一限制性氨基酸。为优化赖氨酸的测定方法,设置了本试验,通过超声振荡器取代普通振荡器,进行300W超声功率下酰化用时及染料结合反应用时的研究。结果表明,最佳酰化用时为15 min,染料结合用时为90 min。与国标法相比,优化后的方法酰化用时变长、染料结合用时变短,测定总用时明显缩短,但测定结果和试验精度更高。可见,采用超声波振荡代替传统振荡器振荡能改进赖氨酸的测定方法。另外,采用最优动态聚类法对赖氨酸含量进行分类,能防止人为分类的不确定性,做到分类方案的最优化。     

DMAIC方法在提高汽车起重机钢丝绳质量中的应用

钢丝绳是汽车起重机的重要零部件之一,对吊装作业的安全性起着关键的作用。通过实施DMAIC方法可降低产品故障的市场反馈率,提高客户满意度。文章首先对钢丝绳质量故障进行定义,明确改善目标;其次利用SIPOC流程分析钢丝绳生产过程,分析影响钢丝绳质量的关键因子并加以改进;最后对改善效果进行验证,固化改进成果。     

Performance and uncertainty analysis of a short-term climate reconstruction based on multi-source data in the Tianshan Mountains region,China

Short-term climate reconstruction, i.e., the reproduction of short-term(several decades) historical climatic time series based on the relationship between observed data and available longer-term reference data in a certain area, can extend the length of climatic time series and offset the shortage of observations. This can be used to assess regional climate change over a much longer time scale. Based on monthly grid climate data from a Coupled Model Inter-comparison Project phase 5(CMIP5) dataset for the period of 1850–2000, the Climatic Research Unit(CRU) dataset for the period of 1901–2000 and the observed data from 53 meteorological stations located in the Tianshan Mountains region(TMR) of China during the period of 1961–2011, we calibrated and validated monthly average temperature(MAT) and monthly accumulated precipitation(MAP) in the TMR using the delta, physical scaling(SP) and artificial neural network(ANN) methods. Performance and uncertainty during the calibration(1971–1999) and verification(1961–1970) periods were assessed and compared using traditional performance indices and a revised set pair analysis(RSPA) method. The calibration and verification processes were subjected to various sources of uncertainty due to the influence of different reconstructed variables, different data sources, and/or different methods used. According to traditional performance indices, both the CRU and CMIP5 datasets resulted in satisfactory calibrated and verified MAT time series at 53 meteorological stations and MAP time series at 20 meteorological stations using the delta and SP methods for the period of 1961–1999. However, the results differed from those obtained by the RSPA method. This showed that the CRU dataset produced a low degree of uncertainty(positive connection degree) during the calibration and verification of MAT using the delta and SP methods compared to the CMIP5 dataset. Overall, the calibrated and verified MAP had a high degree of uncertainty(negative connection degree) regardless of the dataset or reconstruction method used. Therefore, the reconstructed time series of MAT for the period of 1850(or 1901)–1960 based on the CRU and CMIP5 datasets using the delta and SP methods could be used for further study. The results of this study will be useful for short-term(several decades) regional climate reconstruction and longer-term(100 a or more) assessments of regional climate change.     

不同运输条件对合浦珠母贝稚贝存活比较

比较运输方式及环境温度对合浦珠母贝稚贝运输中的存活影响,从而为稚贝运输过程中的运输方式、环境温度及运输时间的把握提供借鉴和参考。以3月龄稚贝为实验材料,运用无水干露、加水淹没及包装充氧等3种方式,分别在环境温度为5、13、21和29℃下模拟运输。每隔3 h从每组运输条件下取出1组实验贝,转移到正常养殖环境下暂养恢复,经48 h后统计其存活率。对运输时间与存活率之间进行非线性参数曲线拟合,计算每组运输条件下的致死时间(T_0)、半致死时间(T_(0.5))及全致死时间(T_1);运用双因素方差分析,Duncan氏方法比较3种运输方式及4种环境温度间的均值。结果显示:包装充氧优于其他两种运输方式,而加水淹没又优于无水干露;温度为13℃和21℃时优于其他2个环境温度,环境温度为17.60～20.05℃是稚贝运输的最佳温度。     

马铃薯淀粉加工的副产物及资源化利用现状

马铃薯淀粉加工过程中会产生大量的副产物,如汁水和薯渣等,这些副产物中有机物含量较高,且COD(化学需氧量)、BOD(生物需氧量)均超标,无法直接排放,作为废弃物又会造成资源的浪费。为减少资源浪费和环境污染,近年对马铃薯淀粉加工副产物处理及资源化利用的研究成为热点。文章主要介绍了马铃薯淀粉的特性、用途、加工过程中副产物的来源,并对目前马铃薯淀粉加工副产物的处理方法及资源化利用现状进行了综述。     

高含固率木质纤维素厌氧发酵物总DNA的4种提取方法比较

不同的样本特性和提取方法对获得微生物总DNA的质量有重要影响。文章基于高含固率木质纤维素厌氧发酵物腐殖酸、酚类物质含量高、质地均一性差、微生物浓度低的特点,研究了4种方法提取不同高含固率粪秸厌氧发酵物中微生物总DNA的效果。结果表明,常规的十二烷基磺酸钠法(sodium dodecyl sulfate,SDS)、十二烷基磺酸钠和溴化十六烷基三甲铵结合法(sodium dodecyl sulfate and cetyltrimethyl ammonium bromide,SDS-CTAB)和商业的粪便试剂盒法提取的DNA质量均较差,SDS法和试剂盒法未能获得聚合酶链式反应(polymerase chain reaction,PCR)扩增目的条带,SDS-CTAB法得到的条带较模糊;改进SDS-CTAB法获得的DNA杂质少、纯度高,具有较好的稳定性,A260/A280和A260/A230值分别为1.74~1.86和1.65~1.86,每克样品的DNA浓度在50 ng·μL^-1以上,电泳条带单一齐整、清晰明亮,PCR扩增的目的条带清晰度高,适宜后续分子生物学技术的分析。林格氏液洗脱、聚乙烯吡咯烷酮-40(Polyvinyl Pyrrolidone-40,PVP-40)洗涤液除杂以及裂解液和多种酶联合破壁是改进SDS-CTAB法获得该类专一性样本高质量微生物总DNA的关键步骤。     

Evaluation of Intestinal Absorption of Dietary Halocynthiaxanthin,a Carotenoid from the Sea Squirt Halocynthia roretzi

Halocynthiaxanthin is an acetylenic carotenoid mainly found in Halocynthia roretzi. To date, several bioactivities of halocynthiaxanthin have been reported, but its mechanism of digestion and absorption in mammals has not been studied yet. In this study, we evaluated the intestinal absorption of halocynthiaxanthin in mice. The halocynthiaxanthin-rich fraction was prepared from the tunicate Halocynthia roretzi. Mice were orally administered the fraction at a dose of 5 mg/kg body weight. The halocynthiaxanthin levels in the plasma, liver, and small intestine, were quantified using HPLC-PDA, 1, 3, 6, and 9 h after ingestion. The halocynthiaxanthin-rich fraction mainly consisted of the all-trans form and a small amount of cis forms. These three isomers were detected in the plasma of mice 3 h after ingestion. Time-course changes after the ingestion of this fraction were found, with cis isomers being more abundant than the all-trans isomer in the mouse plasma and liver. In the small intestine, however, the all-trans isomer was primarily detected. The possibility that cis isomers might be absorbed rapidly from the small intestine cannot be denied, but our results suggest that dietary all-trans-halocynthiaxanthin might be isomerized to the cis isomer after intestinal absorption.